孙宇辉，二级教授、博导、国家重点研发计划首席科学家、英国皇家化学会会士（FRSC）。研究领域为微生物天然产物生物合成机制及合成生物学研究。先后荣获多项国家和省（部）级奖励，目前担任教育部高等学校生物技术、生物工程类专业教学指导委员会委员、The Globe Industrial Microbiology Society国际委员会秘书长、上海交通大学微生物代谢国家重点实验室学术委员会委员、湖北大学生物催化与酶工程省部共建国家重点实验室学术委员会委员、中国微生物学会分子微生物学与生物工程专业委员会委员、湖北省合成生物学学会副理事长、湖北省药学会生化制药专业委员会主任委员、湖北省微生物学会常务理事等。
联系方式
●邮箱：sunyuhui@hust.edu.cn
●网址：http://yhsun.whu.edu.cn
●地址：武汉市硚口区航空路13号同济医学院医学转化大楼1309楼（邮编430030）
研究方向
●微生物来源活性天然产物生物合成途径的解析与药物创新
以具有重要基础和应用价值的微生物天然产物为研究对象，从分子遗传学、生物化学及合成生物学的角度探索其生物合成机理，并回答其生物合成中蕴藏的关键科学问题，为新药创制提供精确的“分子装配线路图”。同时，通过代谢途径的理性设计和定向优化，有的放矢地改造天然产物生物合成途径，实现微生物药物的高产和创新。
● DNA编辑新技术的探究与应用
聚焦于DNA编辑机制的探索和工具的开发，并综合运用DNA编辑技术对靶向基因（簇）进行高效克隆和编辑。
教育与工作经历
教育经历：
● 1988.9―1992.7江西农业大学 攻读植物遗传育种专业学士学位
● 1997.9―2001.3华中农业大学 攻读生物化学与分子生物学专业硕士学位
● 2001.3―2004.3上海交通大学 攻读生物化学与分子生物学专业博士学位
工作经历：
● 1992.6―1998.9江西农业大学生物技术研究开发中心，研究实习员
● 1998.9―2002.10江西农业大学生物技术研究开发中心，助理研究员
● 2002.10―2006.11江西农业大学生物工程系，副教授
● 2006.11―2009.11江西农业大学生物工程与技术学院，教授
● 2005.1―2010.5剑桥大学生物化学系，Royal Society China Fellow、Senior Research Associate
● 2010.5―2023.4武汉大学药学院，教授、湖北省首批“楚天学者特聘教授计划”入选者
● 2023.4―现在 华中科技大学同济医学院药学院，二级教授
主持科研项目
●国家重点研发计划合成生物学重点专项（2020-2024）
●国家自然科学基金重点国际（地区）合作研究项目（2020-2024）
●国家自然科学基金面上项目（2013-2016，2015-2018，2018-2021，2022-2025）
●上海交通大学微生物代谢国家重点实验室开放课题（2015-2017，2018-2020）
●国家重点基础研究发展计划（973）子课题（2012-2016）
●国家高技术研究发展计划（863计划）子课题（2012-2015）
●武汉大学医学部协同创新项目（2013-2015）
●教育部海外名师项目（2012-2017）
●教育部高等学校博士点专项科研基金（博导类）（2012-2014）
●湖北省研究与开发计划项目（2012-2014）
●国家“重大新药创制”科技重大专项子课题（2011-2013）
获奖情况
●湖北省科协优秀科技论文奖（2021-2023）
●湖北省第十六届自然科学优秀学术论文二等奖（2016）
●湖北省第十五届自然科学优秀学术论文一等奖（2014）
●武汉大学优秀烛光导航师（2014）
●第六届武汉大学朱裕璧医学奖（2014）
●国家自然科学二等奖（2008）
●江西省高校科技成果一等奖（2007）
●江西省自然科学二等奖（2006）
●全国优秀博士论文提名奖（2006）
●上海市科学技术进步一等奖（2004）
发表学术论文（*：通讯作者，#：第一作者）
● Jian Wang, Ke Wang, Zhe Deng, Zhiyu Zhong, Guo Sun, Qing Mei, Fuling Zhou, Zixin Deng andYuhui Sun*, Engineered cytosine base editor enabling broad-scope and high-fidelity gene editing inStreptomyces.Nature Communications2024, 15: 5687.
● Xinyun Jian#, Cheng Wang#, Shijuan Wu#, Guo Sun, Chuan Huang, Chengbing Qiu, Yuanzheng Liu, Peter F. Leadlay, Dong Liu, Zixin Deng, Fuling Zhou andYuhui Sun*, Glycodiversification of gentamicins through in vivo glycosyltransferase swapping enabled the creation of novel hybrid aminoglycoside antibiotics with potent activity and low ototoxicity.Acta Pharmaceutica Sinica B2024, https://doi.org/10.1016/j.apsb.2024.04.030.
● Yangning Sun#, Qi Chen#, Yanbing Cheng, Xi Wang, Zixin Deng, Fuling Zhou andYuhui Sun*, Design and engineering of light-induced base editors facilitating genome editing with enhanced fidelity.Advanced Science2023, e2305311.
● Minghe Luo*, Yulu Dong, Zixin Deng andYuhui Sun*, Methylation in hangtaimycin biosynthesis and its antibacterial activities.Synthetic and System Biotechnology2023, 8: 682-687.
● Liang Deng#, Zhihu Zhao#, Lin Liu, Zhiyu Zhong, Wenxinyu Xie, Fan Zhou, Wei Xu, Yubo Zhang, Zixin Deng andYuhui Sun*, Dissection of 3D chromosome organization inStreptomyces coelicolorA3(2) leads to biosynthetic gene cluster overexpression.Proceedings of the National Academy of Sciences of the United States of America2023, 120: e2222045120.
● Guifa Zhai#, Yan Zhu#, Guo Sun, Fan Zhou, Yangning Sun, Zhou Hong, Chuan Dong, Peter F. Leadlay, Kui Hong, Zixin Deng, Fuling Zhou andYuhui Sun*, Insights into azalomycin F assembly-line contribute to evolution-guided polyketide synthase engineering and identification of intermodular recognition.Nature Communications2023, 14: 612.
● Houchao Xu, Anne Wochele, Minghe Luo, Gregor Schnakenburg,Yuhui Sun, Heike Brötz-Oesterhelt and Jeroen S. Dickschat*, Synthesis of tryptophan-dehydrobutyrine diketopiperazine and biological activity of hangtaimycin and its co-metabolites.Beilstein Journal of Organic Chemistry2022, 18: 1159-1165.
● Sicong Li#, Priscila Dos Santos Bury#, Fanglu Huang#, Junhong Guo, Guo Sun, Anna Reva, Chuan Huang, Xinyun Jian, Yuan Li, Jiahai Zhou, Zixin Deng, Finian J. Leeper*, Peter F. Leadlay*, Marcio V. B. Dias* andYuhui Sun*, Mechanistic insights into dideoxygenation in gentamicin biosynthesis.ACS Catalysis2021, 11: 12274-12283.
● Minghe Luo#, Houchao Xu#, Yulu Dong, Kun Shen, Junlei Lu, Zhiyong Yin, Miaomiao Qi, Guo Sun, Lingjie Tang, Jin Xiang, Zixin Deng, Jeroen S. Dickschat* andYuhui Sun*, The mechanism of dehydrating bimodules intrans-acyltransferase polyketide biosynthesis:ashowcase study on hepatoprotective hangtaimycin.Angewandte Chemie International Edition2021, 60: 19139-19143.
● Zhiwei Hu#, Yannan Wang#, Qian Liu, Yan Qiu, Zhiyu Zhong, Kangdi Li, Wenhua Li, Zixin Deng andYuhui Sun*, Improving the precision of base editing by bubble hairpin single guide RNA.mBio2021, 12(2): e 00342-21.
● Zikang Zhou#, Hongzhi Tang#,*, Weiwei Wang, Lige Zhang, Fei Su, Yuanting Wu, Linquan Bai, Sicong Li,Yuhui Sun, Fei TaoandPing Xu*, A cold shock protein promotes high-temperature microbial growth through binding to diverse RNA species.Cell Discovery2021, 7: 15.
● Minghe Luo, Yulu Dong, Lingjie Tang, Xu Chen, Zhiwei Hu, Wenxinyu Xie, Zixin Deng andYuhui Sun*, Two new streptovaricin derivatives from mutants ofStreptomyces spectabilisCCTCC M2017417.Natural Product Research2021, 36: 3689-3694.
● Guo Sun, Chaoqun Hu, Qing Mei, Minghe Luo, Xu Chen, Zhengyuan Li, Yuanzhen Liu, Zixin Deng, Zhengyu Zhang* andYuhui Sun*, Uncovering the cytochrome P450-catalyzed methylenedioxy bridge formation in streptovaricins biosynthesis.Nature Communications2020, 11: 4501.
● Guifa Zhai#, Wenyan Wang#, Wei Xu, Guo Sun, Chaoqun Hu, Xiangming Wu, Zisong Cong, Liang Deng, Yanrong Shi, Peter F. Leadlay, Heng Song, Kui Hong, Zixin Deng andYuhui Sun*, Cross-module enoylreduction in the azalomycin F polyketide synthase.Angewandte Chemie International Edition2020, 59: 22738-22742.
● Minghe Luo, Lingjie Tang, Yulu Dong, Hongbo Huang, Zixin Deng andYuhui Sun*, Antibacterial natural products lobophorin L and M from the marine-derivedStreptomycessp. 4506.Natural Product Research2020, 1-7.
● Yuanzhen Liu*, Xu Chen, Zhengyuan Li, Lixia Huang andYuhui Sun*, Ansavaricin J, a new heterocyclic ring-fused streptovaricin from genestvP5-deleted mutant ofStreptomyces spectabilisCCTCC M2017417.Chemistry & Biodiversity2020, 17: e1900713.
● Yao Liu#, Yuanzhen Liu#, Zixiu Du#, Lidan Zhang, Juan Chen, Zhen Shen, Qian Liu, Juanxiu Qin, Huiying Lv, Hua Wang, Lei He, Junlan Liu, Qian Huang,Yuhui Sun*, Michael Otto* and Min Li*, Skin microbiota analysis-inspired development of novel anti-infectives.Microbiome2020, 8: 85.
● Sicong Li#, Qian Liu#, Zhiyu Zhong, Zixin Deng andYuhui Sun*, Exploration of hygromycin B biosynthesis utilizing CRISPR-Cas9-associated base editing.ACS Chemical Biology2020, 15: 1417-1423.
● Rory Little, Fernanda C. R. Paiva, Robert Jenkins, Hui Hong,Yuhui Sun, Yuliya Demydchuk, Markiyan Samborskyy, Manuela Tosin, Finian J. Leeper, Marcio V. B. Dias and Peter F. Leadlay*, Unexpected enzyme-catalysed [4+2] cycloaddition and rearrangement in polyether antibiotic biosynthesis.Nature Catalysis2019, 2: 1045-1054.
● Weixin Tao*, Li Chen, Chunhua Zhao, Jing Wu, Dazhong Yan, Zixin Deng andYuhui Sun*, In vitro packaging mediated one-step targeted cloning of natural product pathway.ACS Synthetic Biology2019, 8: 1991-1997.
● Zhiyu Zhong#, Junhong Guo#, Liang Deng, Li Chen, Jian Wang, Sicong Li, Wei Xu, Zixin Deng andYuhui Sun*, Base editing inStreptomyceswith Cas9-deaminase fusions.bioRxiv2019 (doi: https://doi.org/10.1101/630137).
● Weixin Tao, Anna Yang, Zixin Deng andYuhui Sun*, CRISPR/Cas9-based editing of streptomyces for discovery, characterization, and production of natural products.Frontiers in Microbiology2018, 9: 1660.
● Sicong Li#, Jun Zhang#, Yuanzhen Liu, Guo Sun, Zixin Deng andYuhui Sun*, Direct genetic and enzymatic evidence for oxidative cyclization in hygromycin B biosynthesis.ACS Chemical Biology2018, 13: 2203-2210.
● Yuan Li#, Wan Zhang#, Hui Zhang, Wenya Tian, Lian Wu, Shuwen Wang, Mengmeng Zheng, Jinru Zhang, Chenghai Sun, Zixin Deng,Yuhui Sun, Xudong Qu* and Jiahai Zhou*, Structural basis of a broadly selective acyltransferase from the polyketide synthase of splenocin.Angewandte Chemie International Edition2018, 57: 5823-5827.
● Sicong Li#, Junhong Guo#, Anna Reva, Fanglu Huang, Binbin Xiong, Yuanzhen Liu, Zixin Deng, Peter F. Leadlay* andYuhui Sun*, Methyltransferases of gentamicin biosynthesis.Proceedings of the National Academy of Sciences of the United States of America2018, 115:1340-1345.
● Yuanzhen Liu, Xu Chen, Zhengyuan Li, Wei Xu, Weixin Tao, Jie Wu, Jian Yang, Zixin Deng andYuhui Sun*, Functional analysis of cytochrome P450s involved in streptovaricin biosynthesis and generation of anti-MRSA analogues.ACS Chemical Biology2017, 12: 2589-2597.
● Priscila dos Santos Bury, Fanglu Huang, Sicong Li,Yuhui Sun, Peter F. Leadlay and Marcio Vinicius Bertacine Dias*, Structural basis of the selectivity of GenN, an aminoglycoside N-methyltransferase involved in gentamicin biosynthesis.ACS Chemical Biology2017, 12: 2779-2787.
● Wei Xu#, Guifa Zhai#, Yuanzhen Liu, Yuan Li, Yanrong Shi, Kui Hong, Hui Hong, Peter F. Leadlay, Zixin Deng andYuhui Sun*, An iterative module in the azalomycin F polyketide synthase contains a switchable enoylreductase domain.Angewandte Chemie International Edition2017, 56: 5503-5506.
● Marie E. Yurkovich, Robert Jenkins,Yuhui Sun, Manuela Tosin* and Peter F. Leadlay*, The polyketide backbone of thiolactomycin is assembled by an unusual iterative polyketide synthase.Chemical Communications2017, 53: 2182-2185.
● Judith Havemann, Marie E. Yurkovich, Robert Jenkins, Sophia Harringer, Weixin Tao, Shishi Wen,Yuhui Sun, Peter F. Leadlay and Manuela Tosin*, Chemical probing of thiotetronate bio-assembly.Chemical Communications2017, 53: 1912-1915.
● Hui Hong,Yuhui Sun, Yongjun Zhou, Emily Stephens, Markiyan Samborskyy and Peter F. Leadlay*, Evidence for an iterative module in chain elongation on the azalomycin polyketide synthase.Beilstein Journal of Organic Chemistry2016, 12: 2164-2172.
● Jianzhao Qi, Dan Wan, Hongmin Ma, Yuanzhen Liu, Rong Gong, Xudong Qu,Yuhui Sun, Zixin Deng and Wenqing Chen*, Deciphering carbamoylpolyoxamic acid biosynthesis reveals unusual acetylation cycle associated with tandem reduction and sequential hydroxylation.Cell Chemical Biology2016, 23: 935-944.
● Yujie Geng, Zixin Deng andYuhui Sun*, An insight into the protospacer adjacent motif ofStreptococcus pyogenesCas9 with artificially stimulated RNA-guided-Cas9 DNA cleavage flexibility.RSC Advances2016, 6: 33514-33522.
● Weixin Tao#, Marie E. Yurkovich#, Shishi Wen#, Karen E. Lebe, Markiyan Samborskyy, Yuanzhen Liu, Anna Yang, Yunkun Liu, Yingchen Ju, Zixin Deng, Manuela Tosin,Yuhui Sun* and Peter F. Leadlay*, A genomics-led approach to deciphering the mechanism of thiotetronate antibiotic biosynthesis.Chemical Science2016, 7: 376-385.
● Yunkun Liu#, Weixin Tao#, Shishi Wen, Zhengyuan Li, Anna Yang, Zixin Deng andYuhui Sun*,In vitroCRISPR/Cas9 system for efficient targeted DNA editing.mBio2015, 6(6): e 01714-15.
● Hu Zeng, Shishi Wen, Wei Xu, Zhaoren He, Guifa Zhai, Yunkun Liu, Zixin Deng andYuhui Sun*, Highly efficient editing of the actinorhodin polyketide chain length factor gene inStreptomyces coelicolorM145 using CRISPR/Cas9-CodA(sm) combined system.Applied Microbiology and Biotechnology2015,99: 10575-10585.
● Chuan Huang#, Fanglu Huang#, Eileen Moison, Junhong Guo, Xinyun Jian, Xiaobo Duan, Zixin Deng, Peter F. Leadlay* andYuhui Sun*, Delineating the biosynthesis of gentamicin X2, the common precursor of the gentamicin C antibiotic complex.Chemistry & Biology2015, 22: 251-261.
● Junhong Guo#, Fanglu Huang#, Chuan Huang, Xiaobo Duan, Xinyun Jian, Finian Leeper, Zixin Deng, Peter F. Leadlay* andYuhui Sun*, Specificity and promiscuity at the branch point in gentamicin biosynthesis.Chemistry & Biology2014, 21:608-618.
●Yuhui Sun*, A carbonate architect emerges.Nature Chemical Biology2014, 10:486-487. (Invited Comments)
● Yanling Ma, Wei Xu, Jun Zhang, Sihong Zhang, Kui Hong, Zixin Deng andYuhui Sun*, Nitrilase superfamily aryl acylamidase from the halotolerant mangroveStreptomycessp. 211726.Applied Microbiology and Biotechnology2014, 98: 8583-8590.
● Weixin Tao#, Manghong Zhu#, Zixin Deng andYuhui Sun*, Biosynthesis of tetronate antibiotics: A growing family of natural products with broad biological activities.Science China Chemistry2013, 56: 1364-1371. (Invited Review)
● Chompoonik Kanchanabanca#, Weixin Tao#, Hui Hong, Yajing Liu, Frank Hahn, Markiyan Samborskyy, Zixin Deng,Yuhui Sun* and Peter F. Leadlay*, Unusual acetylation-elimination in the formation of tetronate antibiotics.Angewandte Chemie International Edition2013, 52: 5785-5788.
● Marie E. Yurkovich, Petros A. Tyrakis, Hui Hong,Yuhui Sun, Markiyan Samborskyy, Kohei Kamiya and Peter F. Leadlay*, A late-stage intermediate in salinomycin biosynthesis is revealed by specific mutation in the biosynthetic gene cluster.ChemBioChem2012, 13: 66-71.
● Jeroen S. Dickschat, Olivia Vergnolle, Hui Hong, Stephen Garner, Susanna R. Bidgood, Hannah C. Dooley, Zixin Deng, Peter F. Leadlay* andYuhui Sun*, An additional dehydratase-like activity is required for lankacidin antibiotic biosynthesis.ChemBioChem2011, 12: 2408-2412.
● Yunlong He,Yuhui Sun, Tiangang Liu, Xiufen Zhou, Linquan Bai* and Zixin Deng, Cloning of separate meilingmycin biosynthesis gene clusters by use of acyltransferase-ketoreductase didomain PCR amplification.Applied and Environmental Microbiology2010, 76: 3283-3292.
●Yuhui Sun*, Frank Hahn, Yuliya Demydchuk, James Chettle, Manuela Tosin, Hiroyuki Osada and Peter F. Leadlay*,In vitroreconstruction of tetronate RK-682 biosynthesis.Nature Chemical Biology2010, 6: 99-101.
●Yuhui Sun, Xinyi He, Jingdan Liang, Xiufen Zhou and Zixin Deng*, Analysis of functions in plasmid pHZ1358 influencing its genetic and structural stability inStreptomyces lividans1326.Applied Microbiology and Biotechnology2009, 82: 303-310.
● David H. Kwan#,Yuhui Sun#, Frank Schulz, Hui Hong, Bojana Popovic, Joalice C. C. Sim-Stark, Stephen F. Haydock and Peter F. Leadlay*, Prediction and manipulation of the stereochemistry of enoylreduction in modular polyketide synthases.Chemistry & Biology2008, 15: 1231-1240.
● Yuliya Demydchuk#,Yuhui Sun#, Hui Hong, James Staunton, Jonathan B. Spencer and Peter F. Leadlay*, Analysis of the tetronomycin gene cluster: insights into the biosynthesis of a polyether tetronate antibiotic.ChemBioChem2008, 9: 1136-1145.
●Yuhui Sun, Hui Hong, Fraser Gillies, Jonathan B. Spencer and Peter F. Leadlay*, Glyceryl-S-acyl carrier protein as an intermediate in the biosynthesis of tetronate antibiotics.ChemBioChem2008, 9: 150-156.
● Barbara M. Harvey, Tatiana Mironenko,Yuhui Sun, Hui Hong, Zixin Deng, Peter F. Leadlay, Kira J. Weissman and Stephen F. Haydock*, Insights into polyether biosynthesis from analysis of the nigericin biosynthetic gene cluster inStreptomycessp. DSM4137.Chemistry & Biology2007, 14: 703-714.
●Yuhui Sun, Hui Hong, Markiyan Samborskyy, Tatiana Mironenko, Peter F. Leadlay and Stephen F. Haydock*, Organization of the biosynthetic gene cluster inStreptomycessp. DSM 4137 for the novel neuroprotectant polyketide meridamycin.Microbiology2006, 152: 3507-3515.
● Tiangang Liu, Delin You, Chiara Valenzano,Yuhui Sun, Jialiang Li, Qing Yu, Xiufen Zhou, David E. Cane and Zixin Deng*, Identification of NanE as the thioesterase for polyether chain release in nanchangmycin biosynthesis.Chemistry & Biology2006, 13:945-955.
●Yuhui Sun, Xiufen Zhou, Hui Dong, Guoquan Tu, Min Wang, Bofei Wang and Zixin Deng*, A complete gene cluster fromStreptomyces nanchangensisNS3226 encoding biosynthesis of the polyether ionophore nanchangmycin.Chemistry & Biology2003, 10: 431-441.
●Yuhui Sun, Xiufen Zhou, Guoquan Tu and Zixin Deng*, Identification of a gene cluster encoding meilingmycin biosynthesis among multiple polyketide synthase contigs isolated fromStreptomyces nanchangensisNS3226.Archives of Microbiology2003, 180: 101-107.
●Yuhui Sun, Xiufen Zhou, Jun Liu, Kai Bao, Guiming Zhang, Guoquan Tu, Tobias Kieser and Zixin Deng*, ‘Streptomyces nanchangensis’, a producer of the insecticidal polyether antibiotic nanchangmycin and the antiparasitic macrolide meilingmycin, contains multiple polyketide gene clusters.Microbiology2002, 148: 361-371.
● Xiuhua Pang, Xiufen Zhou,Yuhui Sunand Zixin Deng*, Physical map of the linear chromosome ofStreptomyces hygroscopicus10-22 deduced by analysis of overlapping large chromosomal deletions.Journal of Bacteriology2002, 184: 1958-1965.
● Xiuhua Pang,Yuhui Sun, Liu Jun, Xiufen Zhou and Zixin Deng*, A linear plasmid temperature-sensitive for replication inStreptomyces hygroscopicus10-22.FEMS Microbiology Letters2002, 208: 25-28.
