杜支凤，博士，副教授。主要从事生物药物（核酸/ADC/环肽）的生物分析以及代谢组学、蛋白组学、中药分析、生物无机化学方面的研究。
联系方式：duzhifeng@hust.edu.cn
教育经历
2005.9-2009.6 武汉大学药学专业毕业，获理学学士学位
2009.9-2014.6 中国科学院化学研究所分析化学专业毕业，获理学博士学位，导师：汪福意
工作经历
2014.9–2016.12美国弗吉尼亚联邦大学化学系博士后导师：Nicolas P Farrell
2017.5-至今 华中科技大学药学院药物分析系 副教授
科研项目
1.化学蛋白质组学研究铂类抗癌药物在复杂生物体系中的结合蛋白，项目批准号：21904044，国家自然科学基金，青年科学基金，2020.01-2022.12，项目主持人。
2.新型鼻腔清洗剂开发，企业横向课题，九州通集团，2018.06-2018.12，项目主持人。
3.基于LC-MS技术的核酸药物分析方法开发，企业横向课题，宏韧生物，2024-2028年，项目主持人。
已发表论文
1.Du Z, Luo Q, Yang L, Bing T, Li X, Guo W, Wu K, Zhao Y, Xiong S, Shangguan Det al: Mass Spectrometric Proteomics Reveals that Nuclear Protein Positive Cofactor PC4 Selectively Binds to Cross-Linked DNA by a trans-Platinum Anticancer Complex.Journal of the American Chemical Society2014, 136(8):2948-2951.
2.Du Z, de Paiva REF, Qu Y, Farrell N*: Tuning the reactivity of Sp1 zinc fingers with platinum complexes.Dalton Transactions2016, 45(21):8712-8716.
3.Du Z,de Paiva REF, Nelson K, Farrell NP*: Diversity in Gold Finger Structure Elucidated by Traveling-Wave Ion Mobility Mass Spectrometry.Angewandte Chemie International Edition2017, 56(16):4464-4467.
4.Li S, Jin S, Song C, Jia S, Zhang Y, Feng Y,Du Z*,Jiang H*: The strategy for establishment of the multiple reaction monitoring based characteristic chemical profile of triterpenes in Alismatis rhizoma using two combined tandem mass spectrometers.Journal of Chromatography A2017, 1524:121-134.
5.Wang Y,Du Z*,Zheng W, Wu K, Xu D, Luo Q, Zhao Y, Han J, Liu Y, Wang F*: Deciphering of interactions between platinated DNA and HMGB1 by hydrogen/deuterium exchange mass spectrometry.Dalton Transactions2017, 46(19):6187-6195.
6.Nie J#, Xiao L#, Zheng L#,Du Z#,Liu D, Zhou J, Xiang J, Hou J, Wang X, Fang J*: An integration of UPLC-DAD/ESI-Q-TOF MS, GC–MS, and PCA analysis for quality evaluation and identification of cultivars of Chrysanthemi Flos (Juhua).Phytomedicine2019, 59:152803.
7.Li A#,Du Z#,Liao M, Feng Y, Ruan H*, Jiang H*: Discovery and characterisation of lycorine-type alkaloids in Lycoris spp. (Amaryllidaceae) using UHPLC-QTOF-MS.Phytochemical Analysis2019, 30(3):268-277.
8.Jia S, Wang R, Wu K, Jiang H,Du Z*:Elucidation of the Mechanism of Action for Metal Based Anticancer Drugs by Mass Spectrometry-Based Quantitative Proteomics.Molecules2019, 24(3):581.
9.Yan Y#,Du Z#,Chen C, Li J, Xiong X, Zhang Y*, Jiang H*: Lysophospholipid profiles of apolipoprotein E-deficient mice reveal potential lipid biomarkers associated with atherosclerosis progression using validated UPLC-QTRAP-MS/MS-based lipidomics approach.Journal of Pharmaceutical and Biomedical Analysis2019, 171:148-157.
10.Du Z,Qu Y*, Farrell NP: Intramolecular platinum migration on a peptide in gas phase during collision-induced dissociation.Journal of Inorganic Biochemistry2020, 202:110858.
11.Jia S, Sun Y, Li L, Wang R, Xiang Y, Li S, Zhang Y, Jiang H*,Du Z*: Discrimination of turmeric from different origins in China by MRM-based curcuminoid profiling and multivariate analysis.Food Chemistry2021, 338:127794.
12. Wang, L., Li, S., Li, J., Cheng, Z., Feng, Y., Ouyang, H.,Zhifeng Du*, Jiang, H*. Comprehensive metabolic profiling of Alismatis Rhizoma triterpenes in rats based on characteristic ions and a triterpene database.Journal of Pharmaceutical Analysis, 2021, 11, 96-107.
13.Wang, R., Wei, Y., Wang, M., Yan, P., Jiang, H., &Du, Z*. Interaction of Natural Compounds in Licorice and Turmeric with HIV-NCp7 Zinc Finger Domain: Potential Relevance to the Mechanism of Antiviral Activity.Molecules,2021,26(12), 3563.
14. Wang, J., Tao, J., Jia, S., Wang, M., Jiang, H., &Du, Z*. The Protein-Binding Behavior of Platinum Anticancer Drugs in Blood Revealed by Mass Spectrometry.Pharmaceuticals, 2021, 14(2), 104.
15. Tao, J.; Jia, S.; Wang, M.; Huang, Z.; Wang, B.; Zhang, W.; Wei, Y.; Li, W.; Jiang, H.;Du, Z.*, Systematic Identification of Proteins Binding with Cisplatin in Blood by Affinity Chromatography and a Four-Dimensional Proteomic Method.Journal of Proteome Research2021, 20, 4553-4565.
16. Wang, M.; Wang, R.; Li, L.; Yan, Y.; Jia, S.; Jiang, H.*;Du, Z.*, Quantitative proteomics of plasma and liver reveals the mechanism of turmeric in preventing hyperlipidemia in mice.Food & Function2021, 12, 10484.
17.Yan, P.; Wang, L.; Li, S.; Liu, X.; Sun, Y.; Tao, J.; Ouyang, H.; Zhang, J.;Du, Z.*; Jiang, H.*, Improved structural annotation of triterpene metabolites of traditional Chinese medicine in vivo based on quantitative structure-retention relationships combined with characteristic ions: Alismatis Rhizoma as an example.Journal of Chromatography B2021,123012.
18.Wei, Y.; Sun, Y.; Jia, S.; Yan, P.; Xiong, C.; Qi, M.; Wang, C.;Du, Z*.; Jiang, H.*, Identification of endogenous carbonyl steroids in human serum by chemical derivatization, hydrogen/deuterium exchange mass spectrometry and the quantitative structure-retention relationship.Journal of Chromatography B2023, 1226, 123776.
19. Chen, Y.; Wang, C.; Qi, M.; Wei, Y.; Jiang, H.;Du, Z.*, Molecular targets of cisplatin in HeLa cells explored through competitive activity-based protein profiling strategy.Journal of Inorganic Biochemistry2024, 254, 112518.
20. Yinyu Wei, Chenyue Zhu, Xinyuan Ye, Chenxi Wang, Meiling Qi, Yi Chen,Zhifeng Du*, Sen Li*, Hongliang Jiang*, Identification of bile acids in snake bile by hydrogen/deuterium exchange mass spectrometry and quantitative structure-retention relationship analysis,Journal of Chromatography A2024, 1736, 465384.
21. Qi, M.; Zhu, C.; Chen, Y.; Wang, C.; Ye, X.; Li, S.; Cheng, Z.; Jiang, H*.;Du, Z.*, Site-Specific Stability Evaluation of Antibody–Drug Conjugate in Serum Using a Validated Liquid Chromatography–Mass Spectrometry Method.Journal of Proteome Research2024. 23, 5131−5142
22. Ye, X.; Jiang, L.; Xiong, Y.; Zhang, C.; Wang, C.; Qi, M.; Zhu, C.; Chen, Y.;Du, Z.*; Cheng, Z.*; Jiang, H.*, Automatic identification of oligonucleotide metabolites in complex biological samples using ultra high-performance liquid chromatography high-resolution mass spectrometry combined with the molecule profiler software.Journal of Chromatography B2025, 1254, 124505.
23.Qi, M.; Xiong, Y.; Chen, M.; Zhang, C.; Zhu, C.; Chen, Y.; Wang, C.; Ye, X.; Jiang, L.; Li, S.; Cheng, Z.; Jiang, H.*;Du, Z.*, Reliable Quantification of Conjugated Peptides from T-DM1 In Vivo by Two-Step Enrichment and Internal Standards from Derivatized Analytes.AnalyticalChemistry2025,97, 25, 13522.
24.Wang, C.; Ye, X.; Zheng, S.; Cheng, Z.; Jia, S.; Zhu, C.; Tian, Y.; Jiang, H.*;Du, Z.*, Comprehensive Characterization of Disulfide Bonds in Insulin and Its Metabolites from Liver S9 by a Proteomic Method.Journal of Proteome Research2025, doi:10.1021/acs.jproteome.5c00696.
授权专利
杜支凤，杨成文，贾帅龙，姜宏梁，一种甘草素探针及其制备方法和应用，2018，中国，201810115358.7
奖励
汪福意，罗群，吴魁，赵耀，杜支凤，郭伟，纪丽云，林煜，翟贵金，刘素彦，金属抗肿瘤化合物相互作用组学研究新方法及其应用，2014，中国分析测试协会科学技术奖二等奖。
